His-tag protein purification resin

His-tag protein purification resin is specially designed for purification of His-tag fusion proteins, it can capture His-tag fusion proteins from cell lysis/culture media. Depending on expression systems, you can choose products with different conjugated ligand types: IDA, NTA and Plus, among which, IDA and NTA are suitable for purifying His-protein expressed in E. coli cells, and Plus is suitable for purifying extracellularly expressed products from insect and mammalian cells.

His-tag protein purification resin (GP-IDA) uses super-macroporous polyacrylate beads as matrix, iminodiacetic acid (IDA) as chelating ligand, and conjugates Ni²⁺. This product features low mass transfer resistance and is suitable for capturing His-protein from high viscosity feed, including supernatant of E. coli cell lysis.

His-tag protein purification resin (CP-NTA) uses macroporous polyacrylate beads as matrix and nitrilotriacetic acid (NTA) as chelating ligand, which is resistant to low concentration of reducing agent added in protein solution, including B-mercaptoethanol or dithiothreitol (DTT).

His-tag protein purification resin (HP-Plus) uses polyacrylate beads as matrix and adopts unique metal chelating ligand (Plus) from Bogen (A Duoning company), which can stably adsorb Ni²⁺ without falling off even in solutions containing EDTA; and it can be CIP with NaOH while no need to be re-conjugate Ni²⁺. This product can greatly save time of sample pretreatment and after-process treatment of resin. It is especially suitable for purification of His-tag proteins expressed and secreted eukaryotic cells, including insect and mammalian cells.

Product features

Specifications	His-tag protein purification resin （GP-IDA）	His-tag protein purification resin （GP-NTA）	His-tag protein purification resin （HP-Plus）
Matrix	Super macroporous polyacrylate hard gel	Macroporous polyacrylate hard gel	Polyacrylate hard gel
Ligand	IDA	NTA	Plus
Average particle size	70 μm	70 μm	70 μm
Reducing agent resistance	NO	β-Me≤100 mM DTT≤5mM	EDTA≤50mM
Dynamic binding capacity	≥ 40 mg/ml	≥ 30 mg/ml	≥ 10 mg/ml
Max flow rate	1000 cm/hr	1000 cm/hr	1000 cm/hr
Max pressure	1 MPa	1 MPa	1 MPa
Storage	4-30 ℃（20% ethanol）	4-30 ℃（20% ethanol）	4-30 ℃（20% ethanol）
Features	High protein capacity	Low metal ion leaching rate	Resistant to high concentration of reducing agents

For different purification requirements, Bogen also provides His-tag protein purification resins based on agarose soft gel matrix. Considering different His protein binding ability and transition metal ion selectivity, we also provide IMAC products without chelated metal ions, users can choose to chelate Cu²⁺, Zn²⁺, Co²⁺ and other transition metal ions.

Specifications	His-tag protein purification resin （NTA）	His-tag protein purification resin （IMAC）
Matrix	Agarose soft gel	Agarose soft gel
Ligand	NTA	IDA/NTA/Plus
Average particle size	90 μm	90 μm
Max flow rate	500 cm/hr	500 cm/hr
Max pressure	0.3 MPa	0.3 MPa
Storage	4-8 ℃（20% ethanol）	4-8 ℃（20% ethanol）
Features	Low metal ion leaching rate	The type of metal ion can be freely selected